Hepatocyte apoptosis in dgalnlpsinduced liver injury. Acute hepatic failure is clinically characterized by massive hepatocyte apoptosis and very high patient mortality. Hepatoprotective activity of bacopa monniera on dgalactosamine induced hepatotoxicity in rats bacopa monniera. The present study investigated the protection by dietary spirulina platensis against dgalactosamine dgaln and acetaminophen apapinduced hepatitis in icr mice. Apoptosis plays a role in the normal development of liver. Protective effect of danhong injection on acute hepatic. Protection by dietary spirulina platensis against d. After 36 hours, only 1020% of the original cells were viable, as assessed by trypan blue exclusion. Dgalactosamine is hepatotoxic and is used, alone or in combination with lps, as a model of liver failure in rodents. Effects of acetylbergenin against d galactosamineinduced. Galactosamineinduced cell death in primary cultures of. Modification of the hepatotoxicity of dgalactosamine in. Because bacterial endotoxin activates kupffer cells, the purpose of this study was to clarify the role of kupffer cells in the mechanism ofd.
Lps is a toxic component of the cell walls of gramnegative bacteria and is widely present in the digestive tracts of humans and animals 3. Hepatotoxic effect of dgalactosamine and protective role. Packaging 10 mg in glass bottle 5, 25 g in poly bottle. Nacetyldgalactosamine is the denantiomer of nacetylgalactosamine. It has a role as a human metabolite, an escherichia coli metabolite and a mouse metabolite. Hepg2 cell lines were used for in vitro study and dgalactosamine dgaln induced. The appearance of dgalactosamineinduced hepatitis and generalized edema in. Pdf dgalactosamine is a hepatotoxic agent, which induces diffuse injury of. Ultraperformance liquid chromatography quadrupole timeofflight mass spectrometry uplcqtofms has been used for. An international journal of pharmaceutical science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Lecithin 50 and 100 mgkg b wt was administered for 1 week by oral route.
However, overactivation thereof may lead to hepatocellular damage. Curcumin attenuates dgalactosaminelipopolysaccharide. Lipopolysaccharide lpsdgalactosamine dgaininduced liver injury has been widely used to study the mechanisms of human hepatitis and to examine hepatic protection of compounds. Acetylbergenin was synthesized from acetylation of bergenin, isolated from mallotus japonicus, to increase lipophilic and physiological activities. Oxidative stress and inflammation contributed to the propagation of acute liver injury ali. Thus, the present study was to evaluate the effectiveness of piper cubeba fruits in the amelioration of ccl 4 induced liver injuries and oxidative damage in the rodent model. Thurman1,3 extrahepatic factors, such as increased gut permeability and bacteria from the gut, have been shown to play a role in. We aim to evaluate the protective effects and definite mechanism by which orally administered b. However, treatment with silymarin and liv52 significantly decrease the biochemical markers, as compared to dgalactosamine group. Protective effect of aa treatment on lipopolysaccharide lps dgalactosamine galninduced fulminant hepatic failure. A voucher specimen 32657 was deposited at the herbarium of the college of pharmacy, king saud university, riyadh, saudi arabia. The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galninduced liver damage in rats, compared with that of bergenin reported previously.
Twenty four wistar strain albino rats were used for the investigations. Full text pdf 1764k abstracts references20 citedby5 the role of endotoxin in liver injury induced by dgalactosamine galn was investigated in rats in vivo, or by using isolated rat hepatocytes in vitro. Metabonomic analysis of liver tissue from balbc mice with. Nature and mechanisms of hepatocyte apoptosis induced by d. Hepatoprotective activity of lepidium sativum seeds. In contrast, jnk2 plays a more critical role than jnk1 in inducing hepatocyte apoptosis in a model of con adgalactosamineinduced liver injury.
Role of free fatty acids in the pathogenesis of fatty liver i seymour m. Methodshepatotoxicity was induced in rats using galn. Hepatotoxicity of dgalactosamine in the isolated perfused rat liver. Primary cultures of rat hepatocytes were exposed to 0. Protective effect of wild ginseng cambial meristematic cells on dgalactosamineinduced hepatotoxicity in rats. Modification of the hepatotoxicity of dgalactosamine in the rat by cycloheximide. Extrahepatic factors, such as increased gut permeability and bacteria from the gut, have been shown to play a role ind. Antihyperlipidemic effect of carvacrol on dgalactosamine. We analyzed the molecular mechanism of galnlpsinduced apoptosis of hepatocytes and examined the therapeutic effects of etoposide on galn lpsinduced lethal liver injury. Acute hepatic failure ahf, which leads to an extremely high mortality rate, has become the focus of attention in clinic.
Carvacrol 2methyl5lmethyiethylphenol is a predominant monoterpenic phenol occuring in many essential oils of the family labiatae including, origanum, satureja, thymbra, thymus, and corydothymus species. A mixture of mulberry and silk amino acids protected against d. Hepatotoxicity was induced in wistar rats by intraperitoneal injection of dgaln 400 mgkg in saline in wistar rats. Effects of dgalactosamineinduced acute liver injury on. Therefore, these results suggest that acetylbergenin has hepatoprotective effects against galninduced hepatotoxicity by inhibiting lipid peroxidation and. Systematic strategy for screening and application of. In colectomized rats, galn produced little hepatotoxicity, while it induced severe liver damage by simultaneous administration of. Lepidium sativum l seeds were procured from a local market in riyadh, saudi arabia. Selective uridine triphosphate deficiency induced by d. Ultrastructural features in galactosamineinduced hepatitis. Ameliorative effect of ethanolic extract of roots of. Hepatoprotective action of alcoholic extract of bacopa monniera bme was evaluated on dgalactosamine dgaln induced rat liver toxicity. Effects of cultured acer tegmentosum cell extract against.
Objective of this study was to obtain a better understanding of the mechanism responsible for the dgalactosamine dgaln induced hepatotoxicity and to study the effect of catechin against dgaln induced hepatotoxicity. Sedum sarmentosum is traditionally used to treat various inflammatory diseases in china. Hepatoprotective effects of punica granatum fruit against dgalactosamineinduced hepatotoxicity in rats. This was demonstrated by reducing the elevated levels of biochemical markers and additional histopathological observations have shown that there is an improvement in the structural design of liver due to induced hepatotoxicity. Ragland division of gastroenterology, university of tennessee center for the health sciences, and veterans administration hospital, memphis, tennessee 38163 received october 25, 1977, and in revised forma. Increasing attention has been paid to spirulina for its potential clinical uses. Liver damage was induced by intra peritoneal administration of 400 mgkg b wt dgalactosamine on the. Treatment with boswellia ovalifoliolata root extracts has protected liver from induced hepatotoxicity.
In addition, it was reported that jnk1, but not jnk2, promoted liver cancer progression in a mouse model of chemicalinduced liver carcinogenesis. Frontiers asiatic acid exhibits antiinflammatory and. Ambrex was orally administered for a period of 7 days at dose levels of 250 and 500 mgkg b. Chronic liver disease such as hepatitis is a major health problem worldwide, especially in developing countries. Green tea extract supplement reduces dgalactosamine. Green tea extract supplement reduces dgalactosamineinduced acute liver injury by inhibition of apoptotic and proinflammatory signaling. Dgalactosamine administration, which indicates a high extent of liver injury. The present study was designed to investigate the effect of carvacrol on dgalactosamine dgalninduced hepatotoxicity in rats. Molecular mechanisms of dgalactosaminelipopolysaccharide. Liver transplant for viral hepatitis and fulminant hepatic failure. Hepatoprotective activity of lepidium sativum seeds against d. Liver diseases still represent a major health burden worldwide. Hepatoprotective effects of purple potato extract against d.
Acetylbergenin was administered orally once daily for 7 days and then galn. Protective role of catechin on dgalactosamine induced. As udpgalnac, galnac is the intial olinked sugar to many serine and threonine residues in protein glycosylations. The aim of the present wok the hepatoprotective activity as well as the antioxidant potential of the ethyl acetate stem extract of m. Multiple factors contribute to hepatic failure, including excessive use of alcohol, viral infection, liver transplant failure, the side effects of chemical drugs, and the metabolism of xenobiotics. Attenuation of oxidative stress and hepatotoxicity induced by d. Hepatoprotective activity of salidroside isolated from acer termentosum max on dgalactosamine induced hepatotoxicity in rats. Experimental and molecular patholocy 29, 8291 1978 dgalactosamine hepatotoxicity v. Glycine and uridine prevent dgalactosamine hepatotoxicity in the rat.
The present study was aimed to evaluate the hepatoprotective activity of ethanolic extract of roots of tetracera akara root on dgalactosamine induced hepatotoxicity in wistar rats. Frontiers pretreatment with bacillus cereus preserves. Manual of histology, staining methods of armed forces, institute of pathology. Nacetyldgalactosamine galnac, an aminosugar, is a component of many olinked and nlinked glycan structures. Pdf hepatoprotective activity of lepidium sativum seeds. Pdf a mixture of mulberry and silk amino acids protected. This study investigated the protective effects of s. This offers the possibility to inhibit utpdependent processes in vivo for periods corresponding to the time interval between galactosamine and uridine injection. Hepatoprotective effects of il22 on fulminant hepatic failure induced by dgalactosamine and lipopolysaccharide in mice. Drugs that induce hepatotoxic effects in mammals were injected into the silkworm.
Hepatoprotective activity of lepidium sativum seeds against dgalactosamine lipopolysaccharide induced hepatotoxicity in animal model. The seeds of lepidium sativum were identified by taxonomist in the college of pharmacy, king saud university, riyadh. Dgalactosamine is an amino sugar derivative of dgalactose item no. Pdf hepatoprotective effects of punica granatum fruit against d. Hepatobiliary clearance of labelled mebrofenin in normal and dgalactosamine hclinduced hepatitis rats and the protective effect of turmeric extract. Dgalactosaminelipopolysaccharideinduced hepatotoxicity. Alf is the most severe form of acute liver injury ali that typically manifests as hepatic dysfunction, coagulopathy, and encephalopathy. It is a nacetyldhexosamine and a nacetylgalactosamine. Against dgalactosamine induced hepatotoxicity in mice. Hepatoprotective effect of pentoxifylline against dgalactosamine. Hepatoprotective activity of lepidium sativum seeds against dgalactosaminelipopolysaccharide induced hepatotoxicity in animal model. Metabolomics, a branch of systems biology, is an important platform in the postgenomics era that focuses on small endogenous metabolites that are the final products of biological metabolite pathways nicholson and lindon 2008. Compared with biofluids, target tissues and organs more directly reflect the pathophysiological state of a disease process.
In fact, an experimental model of liver failure induced by d. The present study was undertaken to determine whether dgalactosamine dgaln induces ali via the mitochondrial apoptosis and proinflammatory cytokinesignaling pathways, and possible mechanisms by which green tea gt extract modulates the apoptotic and proinflammatory signaling. The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharideinduced hepatotoxicity of dgalactosamine sensitized rats. In this study, danhong injection dhi was investigated to evaluate the preventive and protective effect on ahf induced by lipopolysaccharide lps and dgalactosamine galn in mice. To evaluate the hepatoprotective effect of ambrex, a polyherbal formulation against dgalactosamine dgln induced hepatotoxicity in swiss albino. Hepatoprotective activity of seeds against dgalactosamine. Dgalactosamineinduced utp deficiency is reversed completely within 90 min after uridine administration. Glycine and uridine prevent dgalactosamine hepatotoxicity. Hepatoprotective effects of salidroside on fulminant. Our previous studies suggest silkworms can be used as model animals instead of mammals in pharmacologic studies to develop novel therapeutic medicines. The present study was conducted to investigate effect of pentoxifylline ptx on acute liver injury caused by galactosamine dgal in rats and the underlying.
Evaluation of druginduced tissue injury by measuring. Jci insight mitochondrial dnaenriched microparticles. Treatment of experimental animals with dgalactosamine galn lipopolysaccharide lps causes lethal liver injury that is characterized by apoptosis of the hepatocyte. It has protective effects against acute liver injury, but the exact mechanism of such effects remains unclear. Pdf hepatotoxic effect of dgalactosamine and protective role of. In the absence of galactosamine, there was no loss of viability over this same period. Mice in each group n 6 were fed with a standard diet american institute of nutrition ain93g, a positive control diet containing 05 %. Hepatoprotective activity of nigella sativa extract in d. Dgalnlps treatment was able to induce hepatotoxicity and significantly increase all markers of liver damage and lipid peroxidation.
Pretreatment with eeib and silymarin for 21days protected the rat livers from dgalactosamine induced hepatotoxicity. In this study, a dgalactosamine galn lipopolysaccharide lpsinduced mouse model was constructed to investigate metabonomics of liver tissue and directly characterize metabolic changes in acute liver failure alf. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. The aim of this study was to assess dgalactosamine dgalnlipopolysaccharide lpsinduced hepatocyte apoptotic changes in mice and clarify the mechanisms involved in this process. We examined the usefulness of the silkworm larvae bombyx mori as an animal model for evaluating tissue injury induced by various cytotoxic drugs. Galn, a wellknown hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals. This study examined the protective mechanism of wild ginseng cmcs against dgalactosamine galninduced liver injury. The hepatoprotective effects of acetylbergenin were examined against dgalactosamine galn induced liver damage in rats, compared with that of bergenin reported previously. Read hepatoprotective effects of salidroside on fulminant hepatic failure induced by d. Hepatoprotective activity of seeds against d galactosaminelipopolysaccharide induced hepatotoxicity in animal model. Role of endotoxin in dgalactosamine induced hepatic injury. Twentyone spraguedawley rats were equally assigned into three groups n 7 animals per group.